vectastain abc hrp kit (peroxidase, mouse igm ) (Vector Laboratories)

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Vector Laboratories vectastain abc hrp kit (peroxidase, mouse igm )
Vectastain Abc Hrp Kit (Peroxidase, Mouse Igm ), supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 43 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/PK-4010/VECTASTAIN+ABC+HRP+Kit+(Peroxidase%2C+Mouse+IgM+)/custom%40pk-4010%4040199915
Average 93 stars, based on 43 article reviews

vectastain abc hrp kit (peroxidase, mouse igm ) - by Bioz Stars, 2026-11

93/100 stars

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Avidin-Biotin Assay:

Article Title: Simultaneous Treatment of 5-Aminosalicylic Acid and Treadmill Exercise More Effectively Improves Ulcerative Colitis in Mice
Article Snippet: These sections were treated with mouse anti-cleaved caspase-3 (9661S) and anti-cleaved caspase-9 antibodies (9507S, Cell Signaling Technology Inc., Danvers, MA, USA) diluted 1:200 overnight. .. These sections were treated with biotinylated anti-rabbit (BA-1000) and anti-mouse secondary antibodies (BA-2000, Vector Laboratories, Burlingame, CA, USA) for 1 h. These sections were then treated with avidin-biotin-peroxidase complex (PK4010, Vector Laboratories) at room temperature for 1 h. By treating these sections with the solution containing 0.05% 3,3-diaminobenzidine and 0.01% H 2 O 2 in 50 mM Tris-buffer (pH 7.6) for approximately 3 min, immunoreactivity was visualized. .. After counterstaining with Mayer hematoxylin (DAKO), these sections were mounted on gelatin-coated slides, air-dried overnight at room temperature, and coverslipped by Permount ® (Thermo Fisher Scientific).

Article Title: Shear-Sensing by C-Reactive Protein: Linking Aortic Stenosis and Inflammation
Article Snippet: Compound | Sakura Finetek USA (sakuraus.com) M.O.M.® (Mouse on Mouse) Immunodetection Kit Vector Laboratories Mouse on Mouse (M.O.M.) .. Detection Kit, Basic (vectorlabs.com) DAB Substrate Kit, Peroxidase (HRP), with Nickel, (3,3'-diaminobenzidine) Vector Laboratories DAB Staining, DAB Peroxidase Substrate Kit | Vector Labs Mayer′s Hematoxylin Solution Sigma-Aldrich Hematoxylin Solution, Mayer’s Product (sigmaaldrich.com) Proteinase K Agilent S302080-2 | Agilent 3% Hydrogen peroxide solution Millipore https://www.sigmaaldrich.com/AU/en/product/sial/88597 VECTASTAIN® ABC-HRP Kit, Peroxidase Vector Laboratories VECTASTAIN ABC HRP Kit (Peroxidase, Mouse IgM ) (vectorlabs.com) Avidin/Biotin Blocking Kit Vector Laboratories Avidin/Biotin Blocking Kit - VectorLabs CountBrightTM Absolute Counting Beads, for flow cytometry Invitrogen CountBrightTM Absolute Counting Beads, for flow cytometry (thermofisher.com) Submicron Bead Calibration Kit (0.8μm) Bangs Laboratories Product Selection | Bangs Laboratories, Inc. (bangslabs.com) Micron Bead Calibration Kit (1.0μm) Bangs Laboratories Product Selection | Bangs Laboratories, Inc. (bangslabs.com) Phorbol 12-myristate 13-acetate (PMA) Sigma-Aldrich PMA for use in molecular biology applications, = 99 HPLC 16561-29-8 (sigmaaldrich.com) EGMTM-2 Endothelial Cell Growth Medium-2 BulletKitTM (Endothelial Basal Medium and SingleQuots KitTM) Lonza EGM-2 Endothelial Cell Growth Medium-2 BulletKit, CC3162 | Lonza DOI [to be added] 4', 6-diamidino-2-phenylindole, dihydrochloride (DAPI) Cell Signaling Technology DAPI | Cell Signaling Technology Apyrase from potato Sigma-Aldrich Apryase from potato recombinant expressed in Pichia pastoris Sigma (sigmaaldrich.com) Adenosine 5′-diphosphate sodium salt (ADP) Sigma-Aldrich Adenosine 5 -diphosphate bacterial No, = 95 HPLC 20398- 34-9 (sigmaaldrich.com) Rainbow Calibration Particles, 8 peaks (3.0- 3.4 μm) BioLegend https://www.biolegend.com/en-us/products/rainbowcalibration-particles-8-peaks-3-0-3-4-m-8111 DOI [to be added] ..

Article Title: Simultaneous Treatment of 5-Aminosalicylic Acid and Treadmill Exercise More Effectively Improves Ulcerative Colitis in Mice.
Article Snippet: These sections were treated with mouse anti-cleaved caspase-3 (9661S) and anti-cleaved caspase9 antibodies (9507S, Cell Signaling Technology Inc., Danvers, MA, USA) diluted 1:200 overnight. .. These sections were treated with biotinylated anti-rabbit (BA-1000) and antimouse secondary antibodies (BA-2000, Vector Laboratories, Burlingame, CA, USA) for 1 h. These sections were then treated with avidin-biotin-peroxidase complex (PK4010, Vector Laboratories) at room temperature for 1 h. By treating these sections with the solution containing 0.05% 3,3-diaminobenzidine and 0.01% H2O2 in 50 mM Tris-buffer (pH 7.6) for approximately 3 min, immunoreactivity was visualized. .. After counterstaining with Mayer hematoxylin (DAKO), these sections were mounted on gelatin-coated slides, air-dried overnight at room temperature, and coverslipped by Permount® (Thermo Fisher Scientific).

Staining:

Article Title: Induction of Cyp2e1 contributes to asparaginase-induced hepatocyte sensitization to lipotoxicity
Article Snippet: Paraffin sections were deparaffinized, rehydrated, and stained using an anti-4-hydroxynonenal antibody (4-HNE, Thermofisher Scientific, BS-6313R) overnight at 4 °C. .. The VECTASTAIN ABC Kit and DAB Peroxidase Substrate Kit (Vector Laboratories, PK-4010 and SK-4105, respectively) were used to visualize the staining. ..

Article Title: Induction of Cyp2e1 contributes to asparaginase-induced hepatocyte sensitization to lipotoxicity
Article Snippet: Immunostaining and TUNEL analysis Jo urn al P e-p roo f Paraffin sections were deparaffinized, rehydrated, and stained using an anti-4-hydroxynonenal antibody (4- HNE, Thermofisher Scientific, BS-6313R) overnight at 4 °C. .. The VECTASTAIN ABC Kit and DAB Peroxidase Substrate Kit (Vector Laboratories, PK-4010 and SK-4105, respectively) were used to visualize the staining. ..

Blocking Assay:

Flow Cytometry:

Selection:

High Performance Liquid Chromatography:

Recombinant:

Incubation:

Article Title: T1-weighted fast fluid-attenuated inversion-recovery sequence (T1-FFLAIR) enables the visualization and quantification of fetal brain myelination in utero
Article Snippet: After washing, the sections were immersed in 0.3% hydrogen peroxide (in a 3:1 mixture of methanol and re-distilled water) for 30 min, washed in phosphate-buffered saline, and then incubated for 2 h in the blocking solution (phosphate-buffered saline containing 5% bovine serum albumin and 0.5% Triton X-100, all from Sigma) at room temperature (RT) to prevent non-specific background staining. .. The incubation with primary antibody mouse anti-myelin basic protein (1:1000; MBP; clone SMI-99, SMI-99P; Covance) lasted for 48 h at 4 °C; sections were then rinsed in phosphate-buffered saline, and further incubated with secondary antibody anti-mouse (Vectastain ABC kit, PK 4010) for 1 h at RT (Vectastain ABC kit; Vector Laboratories). .. Vectastain ABC reagents (streptavidin–peroxidase complex) were used in the subsequent step for 1 h at RT, and rinsed in phosphate-buffered saline for 3 × 10 min, and finally, peroxidase activity was visualized with 3,3′-diaminobenzidine peroxidase substrate (Sigma-Aldrich).

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